@article {20404, title = {The complete chloroplast genome of Cinnamomum camphora and its comparison with related Lauraceae species}, year = {2017}, abstract = {

Cinnamomum camphora, a member of the Lauraceae family, is a valuable aromatic and timber tree that is indigenous to the south of China and Japan. All parts of Cinnamomum camphora have secretory cells containing different volatile chemical compounds that are utilized as herbal medicines and essential oils. Here, we reported the complete sequencing of the chloroplast genome of Cinnamomum camphora using illumina technology. The chloroplast genome of Cinnamomum camphora is 152,570 bp in length and characterized by a relatively conserved quadripartite structure containing a large single copy region of 93,705 bp, a small single copy region of 19,093 bp and two inverted repeat (IR) regions of 19,886 bp. Overall, the genome contained 123 coding regions, of which 15 were repeated in the IR regions. An analysis of chloroplast sequence divergence revealed that the small single copy region was highly variable among the different genera in the Lauraceae family. A total of 40 repeat structures and 83 simple sequence repeats were detected in both the coding and non-coding regions. A phylogenetic analysis indicated that Calycanthus is most closely related to Lauraceae, both being members of Laurales, which forms a sister group to Magnoliids. The complete sequence of the chloroplast of Cinnamomum camphora will aid in in-depth taxonomical studies of the Lauraceae family in the future. The genetic sequence information will also have valuable applications for chloroplast genetic engineering.

}, keywords = {Chloroplast genome, Cinnamomum camphora, Illumina sequencing, Lauraceae, Phylogeny}, doi = {10.7717/peerj.3820}, author = {Chen, Caihui and Zheng, Yongjie and Liu, Sian and Zhong, Yongda and Wu, Yanfang and Li, Jiang and Xu, Li-An and Xu, Meng} } @article {20401, title = {The complete chloroplast genomes of the evergreen tree species Cinnamomum camphora and Cinnamomum parthenoxylon (Laurales: Lauraceae)}, year = {2019}, abstract = {

Complete chloroplast genomes were characterized for two economically important tree species, Cinnamomum camphora and Cinnamomum parthenoxylon. The chloroplast genomes are 152,721 bp (C. camphora) and 152,760 bp (C. parthenoxylon) long, respectively, and both comprise a pair of inverted repeat (IR) regions, separated by a large single-copy (LSC) region and a small single-copy (SSC) region. The same panel of 112 gene species were annotated for both species, including 78 protein-coding (PCG), 30 tRNA, and 4 rRNA gene species. Phylogenetic analysis suggests that the species delimitation and interrelationship of C. camphora and C. parthenoxylon may need further investigation.

}, doi = {10.1080/23802359.2019.1574677}, author = {Wu, Yi and Wei, Wei and Liu, Li and Liu, Gang and Guo, Qi-Qiang and Qian, Zeng-Qiang} } @article {20397, title = {Incongruence of morphological determinations and DNA barcode sequences: a case study in Cinnamomum (Lauraceae)}, journal = {Willdenowia}, volume = {49}, year = {2019}, pages = {383-400}, chapter = {383}, abstract = {

In the course of molecular systematic studies of Lauraceae we received a sample of a plant cultivated under the name Cinnamomum porrectum in the Botanical Garden M\ünchen-Nymphenburg. Preliminary determinations, both morphologically based on the Flora of China (Li \& al. 2008) and by chloroplast sequences (psbA-trnH spacer, trnK intron including matK gene, trnL intron, trnL-trnF spacer and trnQ-rps16 spacer) obtained by Sanger sequencing suggested that it was C. camphora, still the plant looked different from other individuals of C. camphora cultivated in the botanical gardens of Berlin, Hamburg, Mainz, Munich and Oldenburg. Attempts to sequence the more informative nuclear internal transcribed spacer repeatedly led to mixed signals. We therefore used Illumina sequencing on a set of pre-amplified molecular markers (ITS, trnK 3\′ and 5\′ intron, trnL intron, and the intergenic spacers psbA-trnH, trnL-trnF, as well as parts of the trnQ-rps16 spacer), and downloaded available sequences of C. camphora and C. parthenoxylon from GenBank for comparison. Considerable differences were found among these sequences, but the haplotype groups do not coincide with the current species determinations. Particularly the internal transcribed spacer sequences are rather diverse, suggesting possible misidentifications, contaminations, and/or a common gene pool that is larger than anticipated. Concerning the plant in question, our results suggest that it may be a hybrid, with C. camphora as the maternal and another species, possibly C. parthenoxylon, as the paternal parent.

}, keywords = {Cinnamomum, interspecific hybridization, ITS, Lauraceae, matK, NGS, psbA-trnH spacer, trnK intron, trnL intron, trnL-trnF spacer, trnQ-rps16 spacer}, doi = {10.3372/wi.49.49309}, author = {Rohwer, Jens G. and Trofimov, Dimitrij and Mayland-Quellhorst, Eike and Albach, Dirk} }