@article {20409, title = {ITS and trnH-psbA as efficient DNA barcodes to identify threatened commercial woody angiosperms from southern Brazilian Atlantic Rainforests}, journal = {PLoS ONE}, volume = {10}, year = {2015}, month = {12/2015}, pages = {1-18}, chapter = {1}, abstract = {

The Araucaria Forests in southern Brazil are part of the Atlantic Rainforest, a key hotspot for global biodiversity. This habitat has experienced extensive losses of vegetation cover due to commercial logging and the intense use of wood resources for construction and furniture manufacturing. The absence of precise taxonomic tools for identifying Araucaria Forest tree species motivated us to test the ability of DNA barcoding to distinguish species exploited for wood resources and its suitability for use as an alternative testing technique for the inspection of illegal timber shipments. We tested three cpDNA regions (matK, trnH-psbA, and rbcL) and nrITS according to criteria determined by The Consortium for the Barcode of Life (CBOL). The efficiency of each marker and selected marker combinations were evaluated for 30 commercially valuable woody species in multiple populations, with a special focus on Lauraceae species. Inter- and intraspecific distances, species discrimination rates, and ability to recover species-specific clusters were evaluated. Among the regions and different combinations, ITS was the most efficient for identifying species based on the \‘best close match\’ test; similarly, the trnH-psbA + ITS combination also demonstrated satisfactory results. When combining trnH-psbA + ITS, Maximum Likelihood analysis demonstrated a more resolved topology for internal branches, with 91\% of species-specific clusters. DNA barcoding was found to be a practical and rapid method for identifying major threatened woody angiosperms from Araucaria Forests such as Lauraceae species, presenting a high confidence for recognizing members of Ocotea. These molecular tools can assist in screening those botanical families that are most targeted by the timber industry in southern Brazil and detecting certain species protected by Brazilian legislation and could be a useful tool for monitoring wood exploitation.

}, doi = {10.1371/journal.pone.0143049}, author = {Bolson, M{\^o}nica and Smidt, Eric de C. and Marcelo L Brotto and Silva-Pereira, Viviane} } @mastersthesis {20393, title = {Lauraceae no Parque Estadual das Laur{\'a}ceas - taxonomia, fitossociologia e fitogeografia}, volume = {PhD}, year = {2018}, pages = {233}, school = {Universidade Federal do Paran{\'a}}, address = {Curitiba}, abstract = {

O Parque Estadual das Laur\áceas \é o maior parque estadual no Paran\á, sul do Brasil. Localiza-se nos munic\ípios de Adrian\ópolis, Bocai\úva do Sul e Tunas do Paran\á, em uma \área montanhosa do Primeiro Planalto Paranaense, conhecida como Serra do A\çungui, a qual faz parte da bacia do rio Ribeira. O parque \é quase que totalmente coberto pela Floresta Ombr\ófila Densa e, como seu nome sugere, tem consider\ável presen\ça de \árvores da fam\ília Lauraceae. Apesar disso, faltava informa\ç\ão detalhada sobre esse grupo de plantas na \área. O objetivo principal desse trabalho \é gerar conhecimento sobre suas esp\écies, reunindo informa\ç\ões que possam auxiliar a gest\ão dessa unidade de conserva\ç\ão. No primeiro cap\ítulo n\ós apresentamos o tratamento taxon\ômico das esp\écies que ocorrem no parque e em sua zona de amortecimento. Foi elaborada chave de identifica\ç\ão, descri\ç\ões, dados sobre distribui\ç\ão geogr\áfica e fenologia, pranchas fotogr\áficas, al\ém de coment\ários que auxiliam a identifica\ç\ão de cada uma delas. Constatamos a exist\ência de 51 esp\écies distribu\ídas em dez g\êneros, o que representa a maior riqueza de esp\écies j\á registrada por um estudo taxon\ômico regionalizado para a fam\ília no sul do Brasil. No segundo cap\ítulo n\ós analisamos uma comunidade de Lauraceae dominante em um remanescente muito bem conservado dentro do parque, buscando descrever sua estrutura, e com isso, resgatar a discuss\ão sobre os est\ágios sucessionais das florestas sul brasileiras atrav\és da sua compara\ç\ão com diversos par\âmetros. Para isso, realizamos uma amostragem fitossociol\ógica de dois hectares de floresta. Constatamos que a fam\ília \é capaz de se organizar em uma comunidade complexa inserida na matriz florestal, com v\ários estratos, v\árias idades e v\árias esp\écies, com caracter\ísticas de est\ágio cl\ímax. Conclu\ímos tamb\ém que o parque possui uma associa\ç\ão vegetal tipo Ocotietum, que \é uma tend\ência para os est\ágios sucessionais mais desenvolvidos da Floresta Ombr\ófila Densa Montana subtropical. No terceiro cap\ítulo n\ós revisamos a distribui\ç\ão geogr\áfica das esp\écies que foram identificadas no parque, buscando compreender essa comunidade no contexto brasileiro, em especial no Bioma Mata Atl\ântica. Para isso, realizamos an\álises de similaridade flor\ística com outras 46 comunidades no bioma e tamb\ém an\álises de esp\écies indicadoras. Constatamos a forma\ç\ão de sete grupos flor\ísticos. A comunidade do parque ficou agrupada com outras quinze, todas situadas na regi\ão montanhosa ao longo da costa Atl\ântica, entre os estados do Rio de Janeiro e Santa Catarina. Esse grupo flor\ístico de Lauraceae \é aqui denominado como \“Serra do Mar\”, para o qual foram selecionadas 17 esp\écies indicadoras.

The Laur\áceas State Park is the largest state park in Paran\á, southern Brazil. It is located in the municipalities of Adrian\ópolis, Bocai\úva do Sul, and Tunas do Paran\á, on a mountainous area of Paran\á\’s First Plateau (Primeiro Planalto Paranaense) known as A\çungui mountain range, which is part of the Ribeira river basin. The park is nearly entirely covered by Atlantic Rainforest and, as its name suggests, it has a large presence of trees of the Lauraceae family. Nevertheless, detailed information about this group of plants in the area was lacking. The main goal of the present paper is to generate knowledge about its species, gathering information that may be helpful on the management of this conservation unit. In the first chapter we present the taxonomic treatment of the species that occur in the park and in its buffer zone. Identification keys, descriptions, data on geographic distribution and phenology, and photographic plates are provided, as well as comments that help to identify each species. We found 51 species distributed in ten genera, which represent the greatest species richness recorded by a regionalized taxonomic study for the family in southern Brazil. In the second chapter we analyzed a dominant community of Lauraceae in a very well preserved remnant within the park, aiming to describe its structure, and with this, we bring up the discussion about the succession stages of the Brazilian forests through the comparison of several parameters. For this, we performed a phytosociological sampling of two hectares of forest. We verified that the family is able to organize itself in a complex community inserted in the forest matrix, with several strata, several ages and several species, with characteristics of climax stage. We conclude also that the park has an Ocotietum type of vegetation, which is a tendency for the most developed successional stages of the subtropical Montane Atlantic Rainforest. In the third chapter we review the geographic distribution of the species that were identified in the park, searching to understand this community in the Brazilian context, especially in the Atlantic Forest Biome. For this, we performed analyses of floristic similarity including other 46 communities from the same biome, and also analyses of indicator species. Seven floristic groups were recovered. The park community was grouped with fifteen others, all located in the mountainous region along the Atlantic Ocean, between the states of Rio de Janeiro and Santa Catarina. This particular floristic group of Lauraceae is here named \"Serra do Mar\", for which 17 indicator species were selected.

}, keywords = {Atlantic Rainforest, Biogeography, Botany, Ecology, Forestry succession}, author = {Marcelo L Brotto} } @article {20392, title = {Riqueza e endemismo de Lauraceae no Paran{\'a}: aspectos fitogeogr{\'a}ficos e {\'a}reas priorit{\'a}rias para a conserva{\c c}{\~a}o}, journal = {Rodrigu{\'e}sia}, volume = {70}, year = {2019}, pages = {1-14}, abstract = {

Lauraceae est\á entre as tr\ês principais fam\ílias do componente arb\óreo das florestas no Paran\á, sendo elemento frequente nos estudos fitossociol\ógicos e biogeogr\áficos. Apesar disso, a fam\ília continua insuficientemente conhecida no estado. Nossos objetivos foram: reconhecer as esp\écies de Lauraceae no Paran\á, relacionando-as com as unidades fitogeogr\áficas; mapear as regi\ões com maior riqueza de esp\écies e concentra\ç\ão de endemismos; identificar \áreas de complementaridade e compar\á-las com as \áreas priorit\árias para conserva\ç\ão. Para isso, n\ós utilizamos registros de cole\ç\ões de 23 herb\ários nacionais, totalizando 3.332 esp\écimes analisados, plotando suas coordenadas no mapa fitogeogr\áfico do estado. Constatamos que no Paran\á ocorrem 76 esp\écies, das quais onze s\ão citadas pela primeira vez no estado. A Floresta Ombr\ófila Densa abrange o maior n\úmero de esp\écies (59 spp.), seguida pela Floresta Ombr\ófila Mista (31 spp.), Floresta Estacional Semidecidual (23 spp.), Savana (9 spp.) e Estepe (2 spp.). As regi\ões da Serra do Mar e Vale do Ribeira apresentam a maior riqueza e endemismo. O Parque Estadual das Laur\áceas \é uma unidade de conserva\ç\ão estrat\égica, pois abriga dois ter\ços das esp\écies paranaenses, com sete end\êmicas.

Lauraceae are among the three main families of the arboreal component of the forests in Paran\á, being a frequent element in phytosociological and biogeographic studies. In spite of this, the family remains insufficiently known in the state. Our aims were: to recognize the species of Lauraceae in Paran\á, relating them to the phytogeographic units; map the regions with the greatest species richness and concentration of endemisms; identify complementarity areas and compare them with the priority areas for conservation. We used collection records from 23 national herbaria, totaling 3,332 specimens analyzed, plotting their geographic coordinates onto the phytogeographic map of the state. We found that there are 76 species in Paran\á, of which eleven are cited for the first time in the state. The Atlantic Rainforest contains the largest number of species (59 spp.), followed by the Araucaria Rainforest (31 spp.), Semideciduous Forest (23 spp.), Savanna (9 spp.), and Grasslands (2 spp.). The Serra do Mar and Ribeira Valley regions have the highest concentration of species, as well as the highest index of endemism. The Laur\áceas State Park is a strategic conservation unit, because it houses two thirds of the species of Paran\á, including seven endemic.

}, keywords = {Atlantic Forest, Biogeography, complementarity, endemism, priority areas}, doi = {10.1590/2175-7860201970044}, author = {Marcelo L Brotto and Uhlmann, Alexandre and Blum, Christopher T and Roderjan, Carlos V} } @article {20352, title = {Nova esp{\'e}cie de Ocotea Aubl. para a regi{\~a}o neotropical, estados de S{\~a}o Paulo e Rio de Janeiro, Brasil}, journal = {Heringeriana}, volume = {10}, year = {2016}, pages = {69-77}, chapter = {69}, abstract = {

It describes a new species of Lauraceae for the Neotropics, southeastern Brazil, S\ão Paulo and Rio de Janeiro. Ocotea koscinskii Baitello \& Brotto is an unisexual species very similar to Ocotea dispersa (Nees) Mez, but differs in particular by the globular shape of the fruit, no remaining tepals at the cupule of the fruit and thyrso-paniculate inflorescence. They still differ by occurrences in different strata when coexist in ombrophilous forests, respectively canopy and understory.

}, issn = {1983-6996}, author = {Baitello, Jo{\~a}o B and Marcelo L Brotto} } @article {20207, title = {Lauraceae no Morro dos Perdidos (Floresta Atl{\^a}ntica), Paran{\'a}, Brasil}, journal = {Rodrigu{\'e}sia}, volume = {60}, year = {2009}, pages = {445-459}, chapter = {445}, abstract = {

Morro dos Perdidos is located in Guaratuba, Paran\á State (25\º45\’\–25\º50\’S and 49\º03\’\–49\º06\’W), comprising 1440 hectares of Atlantic Rain Forest, with altitudes between 767 and 1439 m. In the area, the family Lauraceae is represented by 15 species: Cinnamomum hatschbachii; Cryptocarya aschersoniana; Endlicheria paniculata; Nectandra puberula; Ocotea bicolor; O. catharinensis; O. elegans; O. nunesiana; O. odorifera; O. porosa; O. pulchella; O. tristis; O. vaccinioides; Ocotea sp and Persea willdenovii. Key, descriptions, illustrations and comments about the species are presented.

}, author = {Marcelo L Brotto} } @article {19929, title = {Uma esp{\'e}cie nova de Lauraceae da floresta atl{\^a}ntica do Brasil}, journal = {Rodrigu{\'e}sia}, volume = {63}, year = {2012}, pages = {579-585}, chapter = {579}, abstract = {

Uma esp\écie nova de Ocotea Aubl. (Lauraceae), Ocotea marumbiensis Brotto \& Baitello, \é descrita e ilustrada.\  A esp\écie, com flores hermafroditas, assemelha-se a Ocotea indecora (Schott) Mez e ocorre em floresta atl\ântica nos estados do Paran\á e Santa Catarina, Regi\ão Sul do Brasil.

A new species of Ocotea Aubl. (Lauraceae), Ocotea marumbiensis Brotto \& Baitello, is described and illustrated. The species with hermaphrodite flowers resembles Ocotea indecora (Schott) Mez and occurs in the Atlantic Forest in the states of Parana and Santa Catarina, southern Brazil.

}, author = {Marcelo L Brotto and Baitello, Jo{\~a}o B} }