02678nas a2200289 4500008004100000245011500041210006900156300001200225490000700237520182400244653001502068653003202083653000802115653001402123653000902137653000802146653002102154653001602175653001602191653002102207653002202228100002102250700002302271700002902294700001702323856004802340 2019 eng d00aIncongruence of morphological determinations and DNA barcode sequences: a case study in Cinnamomum (Lauraceae)0 aIncongruence of morphological determinations and DNA barcode seq a383-4000 v493 a

In the course of molecular systematic studies of Lauraceae we received a sample of a plant cultivated under the name Cinnamomum porrectum in the Botanical Garden München-Nymphenburg. Preliminary determinations, both morphologically based on the Flora of China (Li & al. 2008) and by chloroplast sequences (psbA-trnH spacer, trnK intron including matK gene, trnL intron, trnL-trnF spacer and trnQ-rps16 spacer) obtained by Sanger sequencing suggested that it was C. camphora, still the plant looked different from other individuals of C. camphora cultivated in the botanical gardens of Berlin, Hamburg, Mainz, Munich and Oldenburg. Attempts to sequence the more informative nuclear internal transcribed spacer repeatedly led to mixed signals. We therefore used Illumina sequencing on a set of pre-amplified molecular markers (ITS, trnK 3′ and 5′ intron, trnL intron, and the intergenic spacers psbA-trnH, trnL-trnF, as well as parts of the trnQ-rps16 spacer), and downloaded available sequences of C. camphora and C. parthenoxylon from GenBank for comparison. Considerable differences were found among these sequences, but the haplotype groups do not coincide with the current species determinations. Particularly the internal transcribed spacer sequences are rather diverse, suggesting possible misidentifications, contaminations, and/or a common gene pool that is larger than anticipated. Concerning the plant in question, our results suggest that it may be a hybrid, with C. camphora as the maternal and another species, possibly C. parthenoxylon, as the paternal parent.

10aCinnamomum10ainterspecific hybridization10aITS10aLauraceae10amatK10aNGS10apsbA-trnH spacer10atrnK intron10atrnL intron10atrnL-trnF spacer10atrnQ-rps16 spacer1 aRohwer, Jens, G.1 aTrofimov, Dimitrij1 aMayland-Quellhorst, Eike1 aAlbach, Dirk uhttps://lauraceae.myspecies.info/node/2039701968nas a2200205 4500008004100000245016400041210006900205260001200274300000900286490001100295520124400306653003201550653002701582653002601609653001401635653002101649100002301670700002101693856004801714 2020 eng d00aTowards a phylogenetic classification of the Ocotea complex (Lauraceae): an analysis with emphasis on the Old World taxa and description of the new genus Kuloa0 aTowards a phylogenetic classification of the Ocotea complex Laur c01/2020 a1-260 vboz0883 a

Ocotea is one of the largest genera in the Lauraceae (c. 400 spp.), and it has been known to be paraphyletic with respect to most other genera of the New World Lauraceae for almost 20 years. In the traditional circumscription, Ocotea contains not only Neotropical species, but also about 45 species from the African region, including Macaronesia, Madagascar, and the Comoro and Mascarene Islands. Only a few of the species have been included in previous molecular systematic analyses. Here we present a phylogenetic analysis, based on ITS and psbA-trnH sequences of 168 Lauraceae species, including 151 taxa from the Ocotea complex, among them 26 of the 45 Palaeotropical species currently placed in Ocotea. Our results show that the Old World species belong to two well-supported and morphologically distinguishable clades, one of which is placed unresolved among the Neotropical clades of the Ocotea complex, whereas the other is sister to Cinnamomum section Cinnamomum. The two clades can also be differentiated based morphologically. As a step towards a phylogenetic classification, we recognize the second group as the new genus Kuloa.

10ainternal transcribed spacer10amorphological analysis10aphylogenetic analysis10apsbA-trnH10astomatal complex1 aTrofimov, Dimitrij1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/2037601637nas a2200241 4500008004100000245013800041210006900179260001200248300001000260490000800270520082900278653003001107653002101137653003201158653002401190653002601214653001401240653002201254100002301276700002701299700002101326856004801347 2019 eng d00aTowards a phylogenetic classification of the Ocotea complex (Lauraceae): classification principles and reinstatement of Mespilodaphne0 aTowards a phylogenetic classification of the Ocotea complex Laur c04/2019 a25-500 v1903 a

The large genus Ocotea (c. 400 species) has been known to be paraphyletic with respect to most other Neotropical genera of Lauraceae for almost 20 years, but a phylogenetic classification has not yet been proposed. Here we present a phylogenetic analysis, based on ITS and psbA-trnH sequences of 123 species from the Ocotea complex, focused on the Neotropical species with bisexual flowers. Our results show that several well-supported clades can be recognized morphologically. We discuss the principles of splitting monophyletic genera from large paraphyletic groups, and we reinstate the genus Mespilodaphne as a first step towards a phylogenetic classification. In addition, three species previously included in Aiouea are transferred to Damburneya.

10aclassification principles10afruit morphology10ainternal transcribed spacer10aparaphyletic groups10aphylogenetic analysis10apsbA-trnH10astamen morphology1 aTrofimov, Dimitrij1 aMoraes, Pedro, L.R. de1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/2037301336nas a2200181 4500008004100000245010600041210006900147260001200216300001000228490000700238520077900245653001201024653001401036653001201050100002301062700002101085856004801106 2018 eng d00aEpidermal features allowing identification of evolutionary lineages in the Ocotea complex (Lauraceae)0 aEpidermal features allowing identification of evolutionary linea c01/2018 a17-350 v313 a

Epidermal characters are widely used in identification of fossil plant material, yet they are insufficiently known in many extant taxa. Here, we examined the cuticle and stomatal complex in 85 species of the Ocotea complex by optical and scanning electron microscopy. We defined 19 types of stomatal surface appearance, which were mostly consistent with genera or species groups recognized based on reproductive characteristics. The epidermal features support the conclusion derived from DNA analyses that Ocotea is heterogeneous in its current circumscription. We suggest an evolution of the subsidiary cells in the Ocotea complex species from asymmetric to symmetric, and from relatively wide to narrower shapes of the stomatal complex.

10acuticle10aevolution10astomata1 aTrofimov, Dimitrij1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/2036902392nas a2200157 4500008004100000245009100041210006900132260001200201300001200213490000700225520188900232100002302121700002102144700002102165856004802186 2016 eng d00aPhylogenetic study of the genus Nectandra (Lauraceae), and reinstatement of Damburneya0 aPhylogenetic study of the genus Nectandra Lauraceae and reinstat c10/2016 a980-9960 v653 a

Earlier studies indicated that the genus Nectandra, currently the second-largest genus of the Neotropical Lauraceae, might be diphyletic in its traditional circumscription, but the evidence was not quite conclusive. Our phylogenetic analyses based on nuclear (ITS) and chloroplast (psbA-trnH) sequences of 45 Nectandra species as well as 42 representatives of 18 genera of the core Lauraceae (Ocotea complex, Laureae, Aiouea, Asian Cinnamomum and Persea groups) confirm the suspicion that Nectandra is diphyletic. The two groups, Nectandra s.str. and the N. coriacea group, are each well supported in the maximum parsimony and Bayesian analyses, but they are not sister to each other. Nectandra s.str. is sister to Pleurothyrium, and the clade including Nectandra s.str. and Pleurothyrium is very likely closer to the dioecious taxa of the Ocotea complex. The N. coriacea group, on the other hand, appears to be closer to bisexual taxa known as the Ocotea helicterifolia group. In addition, Nectandra s.str. is characterized by a large deletion in the psbA-trnH spacer compared to all other core Lauraceae, including its sister group Pleurothyrium. Considering these facts, Nectandra cannot be maintained in the traditional sense. We therefore transfer the species of the N. coriacea group to the oldest generic name applied to this group, Damburneya . On the basis of fossil records from the American subcontinents and in accordance with previous phylogenetic and molecular clock studies we suggest a scenario of a climate-driven migration from North to South America, with a subsequent rapid radiation of Nectandra s.str. in South America.

1 aTrofimov, Dimitrij1 aRudolph, Barbara1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/20346