02297nas a2200241 4500008004100000245010900041210006900150520156400219653002301783653002401806653002401830653001401854653001401868100001701882700001901899700001401918700001801932700001601950700001401966700001401980700001301994856004802007 2017 eng d00aThe complete chloroplast genome of Cinnamomum camphora and its comparison with related Lauraceae species0 acomplete chloroplast genome of Cinnamomum camphora and its compa3 a

Cinnamomum camphora, a member of the Lauraceae family, is a valuable aromatic and timber tree that is indigenous to the south of China and Japan. All parts of Cinnamomum camphora have secretory cells containing different volatile chemical compounds that are utilized as herbal medicines and essential oils. Here, we reported the complete sequencing of the chloroplast genome of Cinnamomum camphora using illumina technology. The chloroplast genome of Cinnamomum camphora is 152,570 bp in length and characterized by a relatively conserved quadripartite structure containing a large single copy region of 93,705 bp, a small single copy region of 19,093 bp and two inverted repeat (IR) regions of 19,886 bp. Overall, the genome contained 123 coding regions, of which 15 were repeated in the IR regions. An analysis of chloroplast sequence divergence revealed that the small single copy region was highly variable among the different genera in the Lauraceae family. A total of 40 repeat structures and 83 simple sequence repeats were detected in both the coding and non-coding regions. A phylogenetic analysis indicated that Calycanthus is most closely related to Lauraceae, both being members of Laurales, which forms a sister group to Magnoliids. The complete sequence of the chloroplast of Cinnamomum camphora will aid in in-depth taxonomical studies of the Lauraceae family in the future. The genetic sequence information will also have valuable applications for chloroplast genetic engineering.

10aChloroplast genome10aCinnamomum camphora10aIllumina sequencing10aLauraceae10aPhylogeny1 aChen, Caihui1 aZheng, Yongjie1 aLiu, Sian1 aZhong, Yongda1 aWu, Yanfang1 aLi, Jiang1 aXu, Li-An1 aXu, Meng uhttps://lauraceae.myspecies.info/node/2040401290nas a2200157 4500008004100000245013800041210006900179520074700248100001100995700001301006700001201019700001401031700001801045700002101063856004801084 2019 eng d00aThe complete chloroplast genomes of the evergreen tree species Cinnamomum camphora and Cinnamomum parthenoxylon (Laurales: Lauraceae)0 acomplete chloroplast genomes of the evergreen tree species Cinna3 a

Complete chloroplast genomes were characterized for two economically important tree species, Cinnamomum camphora and Cinnamomum parthenoxylon. The chloroplast genomes are 152,721 bp (C. camphora) and 152,760 bp (C. parthenoxylon) long, respectively, and both comprise a pair of inverted repeat (IR) regions, separated by a large single-copy (LSC) region and a small single-copy (SSC) region. The same panel of 112 gene species were annotated for both species, including 78 protein-coding (PCG), 30 tRNA, and 4 rRNA gene species. Phylogenetic analysis suggests that the species delimitation and interrelationship of C. camphora and C. parthenoxylon may need further investigation.

1 aWu, Yi1 aWei, Wei1 aLiu, Li1 aLiu, Gang1 aGuo, Qi-Qiang1 aQian, Zeng-Qiang uhttps://lauraceae.myspecies.info/node/2040102678nas a2200289 4500008004100000245011500041210006900156300001200225490000700237520182400244653001502068653003202083653000802115653001402123653000902137653000802146653002102154653001602175653001602191653002102207653002202228100002102250700002302271700002902294700001702323856004802340 2019 eng d00aIncongruence of morphological determinations and DNA barcode sequences: a case study in Cinnamomum (Lauraceae)0 aIncongruence of morphological determinations and DNA barcode seq a383-4000 v493 a

In the course of molecular systematic studies of Lauraceae we received a sample of a plant cultivated under the name Cinnamomum porrectum in the Botanical Garden München-Nymphenburg. Preliminary determinations, both morphologically based on the Flora of China (Li & al. 2008) and by chloroplast sequences (psbA-trnH spacer, trnK intron including matK gene, trnL intron, trnL-trnF spacer and trnQ-rps16 spacer) obtained by Sanger sequencing suggested that it was C. camphora, still the plant looked different from other individuals of C. camphora cultivated in the botanical gardens of Berlin, Hamburg, Mainz, Munich and Oldenburg. Attempts to sequence the more informative nuclear internal transcribed spacer repeatedly led to mixed signals. We therefore used Illumina sequencing on a set of pre-amplified molecular markers (ITS, trnK 3′ and 5′ intron, trnL intron, and the intergenic spacers psbA-trnH, trnL-trnF, as well as parts of the trnQ-rps16 spacer), and downloaded available sequences of C. camphora and C. parthenoxylon from GenBank for comparison. Considerable differences were found among these sequences, but the haplotype groups do not coincide with the current species determinations. Particularly the internal transcribed spacer sequences are rather diverse, suggesting possible misidentifications, contaminations, and/or a common gene pool that is larger than anticipated. Concerning the plant in question, our results suggest that it may be a hybrid, with C. camphora as the maternal and another species, possibly C. parthenoxylon, as the paternal parent.

10aCinnamomum10ainterspecific hybridization10aITS10aLauraceae10amatK10aNGS10apsbA-trnH spacer10atrnK intron10atrnL intron10atrnL-trnF spacer10atrnQ-rps16 spacer1 aRohwer, Jens, G.1 aTrofimov, Dimitrij1 aMayland-Quellhorst, Eike1 aAlbach, Dirk uhttps://lauraceae.myspecies.info/node/2039702392nas a2200157 4500008004100000245009100041210006900132260001200201300001200213490000700225520188900232100002302121700002102144700002102165856004802186 2016 eng d00aPhylogenetic study of the genus Nectandra (Lauraceae), and reinstatement of Damburneya0 aPhylogenetic study of the genus Nectandra Lauraceae and reinstat c10/2016 a980-9960 v653 a

Earlier studies indicated that the genus Nectandra, currently the second-largest genus of the Neotropical Lauraceae, might be diphyletic in its traditional circumscription, but the evidence was not quite conclusive. Our phylogenetic analyses based on nuclear (ITS) and chloroplast (psbA-trnH) sequences of 45 Nectandra species as well as 42 representatives of 18 genera of the core Lauraceae (Ocotea complex, Laureae, Aiouea, Asian Cinnamomum and Persea groups) confirm the suspicion that Nectandra is diphyletic. The two groups, Nectandra s.str. and the N. coriacea group, are each well supported in the maximum parsimony and Bayesian analyses, but they are not sister to each other. Nectandra s.str. is sister to Pleurothyrium, and the clade including Nectandra s.str. and Pleurothyrium is very likely closer to the dioecious taxa of the Ocotea complex. The N. coriacea group, on the other hand, appears to be closer to bisexual taxa known as the Ocotea helicterifolia group. In addition, Nectandra s.str. is characterized by a large deletion in the psbA-trnH spacer compared to all other core Lauraceae, including its sister group Pleurothyrium. Considering these facts, Nectandra cannot be maintained in the traditional sense. We therefore transfer the species of the N. coriacea group to the oldest generic name applied to this group, Damburneya . On the basis of fossil records from the American subcontinents and in accordance with previous phylogenetic and molecular clock studies we suggest a scenario of a climate-driven migration from North to South America, with a subsequent rapid radiation of Nectandra s.str. in South America.

1 aTrofimov, Dimitrij1 aRudolph, Barbara1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/2034600411nam a2200097 4500008004100000245005400041210005400095260007000149100002800219856006600247 1843 eng d00aSystema materiae medicae vegetabilis brasiliensis0 aSystema materiae medicae vegetabilis brasiliensis aLeipzig [Lipsiae]; Wien [Vindobonae]bFrid. Fleischer; Frid. Beck1 avon Martius, Carl, F.P. uhttp://www.biodiversitylibrary.org/item/38662#page/3/mode/1up00344nas a2200109 4500008004100000245004400041210004000085300001200125490000700137100002300144856006700167 1912 eng d00aDecades plantarum novarum. LXXV-LXXIX.0 aDecades plantarum novarum LXXVLXXIX a369-3780 v101 aLéveillé, Hector uhttp://www.biodiversitylibrary.org/item/7031#page/375/mode/1up00371nam a2200121 4500008004100000245002600041210002600067260005400093300000800147490000600155100002000161856006800181 1790 eng d00aFlora Cochinchinensis0 aFlora Cochinchinensis aUlyssipone [Lisbon]bTypis et expensis Academicis a3530 v11 aLoureiro, João uhttp://www.biodiversitylibrary.org/item/89540#page/281/mode/1up