01290nas a2200157 4500008004100000245013800041210006900179520074700248100001100995700001301006700001201019700001401031700001801045700002101063856004801084 2019 eng d00aThe complete chloroplast genomes of the evergreen tree species Cinnamomum camphora and Cinnamomum parthenoxylon (Laurales: Lauraceae)0 acomplete chloroplast genomes of the evergreen tree species Cinna3 a

Complete chloroplast genomes were characterized for two economically important tree species, Cinnamomum camphora and Cinnamomum parthenoxylon. The chloroplast genomes are 152,721 bp (C. camphora) and 152,760 bp (C. parthenoxylon) long, respectively, and both comprise a pair of inverted repeat (IR) regions, separated by a large single-copy (LSC) region and a small single-copy (SSC) region. The same panel of 112 gene species were annotated for both species, including 78 protein-coding (PCG), 30 tRNA, and 4 rRNA gene species. Phylogenetic analysis suggests that the species delimitation and interrelationship of C. camphora and C. parthenoxylon may need further investigation.

1 aWu, Yi1 aWei, Wei1 aLiu, Li1 aLiu, Gang1 aGuo, Qi-Qiang1 aQian, Zeng-Qiang uhttps://lauraceae.myspecies.info/node/2040102678nas a2200289 4500008004100000245011500041210006900156300001200225490000700237520182400244653001502068653003202083653000802115653001402123653000902137653000802146653002102154653001602175653001602191653002102207653002202228100002102250700002302271700002902294700001702323856004802340 2019 eng d00aIncongruence of morphological determinations and DNA barcode sequences: a case study in Cinnamomum (Lauraceae)0 aIncongruence of morphological determinations and DNA barcode seq a383-4000 v493 a

In the course of molecular systematic studies of Lauraceae we received a sample of a plant cultivated under the name Cinnamomum porrectum in the Botanical Garden München-Nymphenburg. Preliminary determinations, both morphologically based on the Flora of China (Li & al. 2008) and by chloroplast sequences (psbA-trnH spacer, trnK intron including matK gene, trnL intron, trnL-trnF spacer and trnQ-rps16 spacer) obtained by Sanger sequencing suggested that it was C. camphora, still the plant looked different from other individuals of C. camphora cultivated in the botanical gardens of Berlin, Hamburg, Mainz, Munich and Oldenburg. Attempts to sequence the more informative nuclear internal transcribed spacer repeatedly led to mixed signals. We therefore used Illumina sequencing on a set of pre-amplified molecular markers (ITS, trnK 3′ and 5′ intron, trnL intron, and the intergenic spacers psbA-trnH, trnL-trnF, as well as parts of the trnQ-rps16 spacer), and downloaded available sequences of C. camphora and C. parthenoxylon from GenBank for comparison. Considerable differences were found among these sequences, but the haplotype groups do not coincide with the current species determinations. Particularly the internal transcribed spacer sequences are rather diverse, suggesting possible misidentifications, contaminations, and/or a common gene pool that is larger than anticipated. Concerning the plant in question, our results suggest that it may be a hybrid, with C. camphora as the maternal and another species, possibly C. parthenoxylon, as the paternal parent.

10aCinnamomum10ainterspecific hybridization10aITS10aLauraceae10amatK10aNGS10apsbA-trnH spacer10atrnK intron10atrnL intron10atrnL-trnF spacer10atrnQ-rps16 spacer1 aRohwer, Jens, G.1 aTrofimov, Dimitrij1 aMayland-Quellhorst, Eike1 aAlbach, Dirk uhttps://lauraceae.myspecies.info/node/20397