01290nas a2200157 4500008004100000245013800041210006900179520074700248100001100995700001301006700001201019700001401031700001801045700002101063856004801084 2019 eng d00aThe complete chloroplast genomes of the evergreen tree species Cinnamomum camphora and Cinnamomum parthenoxylon (Laurales: Lauraceae)0 acomplete chloroplast genomes of the evergreen tree species Cinna3 a

Complete chloroplast genomes were characterized for two economically important tree species, Cinnamomum camphora and Cinnamomum parthenoxylon. The chloroplast genomes are 152,721 bp (C. camphora) and 152,760 bp (C. parthenoxylon) long, respectively, and both comprise a pair of inverted repeat (IR) regions, separated by a large single-copy (LSC) region and a small single-copy (SSC) region. The same panel of 112 gene species were annotated for both species, including 78 protein-coding (PCG), 30 tRNA, and 4 rRNA gene species. Phylogenetic analysis suggests that the species delimitation and interrelationship of C. camphora and C. parthenoxylon may need further investigation.

1 aWu, Yi1 aWei, Wei1 aLiu, Li1 aLiu, Gang1 aGuo, Qi-Qiang1 aQian, Zeng-Qiang uhttps://lauraceae.myspecies.info/node/2040100813nas a2200169 4500008004100000245006000041210005600101260001200157300001000169490000700179520034100186653001800527653001200545653001400557100002400571856004800595 2019 eng d00aA new species of Chlorocardium (Lauraceae) from Ecuador0 anew species of Chlorocardium Lauraceae from Ecuador c05/2019 a81-860 v273 a

Chlorocardium esmeraldense van der Werff, a new species from the Pacific coast of Ecuador, is described and illustrated. The circumscription of Chlorocardium Rohwer, H. G. Richt. & van der Werff is widened. The genus now includes three species, one with tetramerous flowers and two with trimerous flowers.

10aChlorocardium10aEcuador10aLauraceae1 avan der Werff, Henk uhttps://lauraceae.myspecies.info/node/2039901689nas a2200121 4500008004100000245008800041210006900129300001100198490000700209520127800216100002501494856004801519 2019 eng d00aA revision of Cinnamomum Schaeff. (Lauraceae) for Peninsular Malaysia and Singapore0 arevision of Cinnamomum Schaeff Lauraceae for Peninsular Malaysia a89-1390 v713 a

A revision of all species of the genus Cinnamomum Schaeff. (Lauraceae) occurring in Peninsular Malaysia and Singapore is presented with a summary of taxonomic history, notable features of morphology, a key to species, and a description, distribution map, Provisional IUCN Conservation Assessment for each species, together with notes on ecology and ethnobotany. In this revision, twenty-two distinct species are recognised, including one former variety that is elevated to species level as Cinnamomum selangorense (Ridl.) de Kok. Eleven names are lectotypified (Camphora inuncta Nees; Cinnamomum cinereum Gamble, C. graciliflorum Gamble, C. malaccense Meisn., C. ridleyi Gamble, C. rhynchophyllum Miq., C. sintoc Blume, C. subcuneatum Miq., C. velutinum Ridl., and C. vimineum Wall. ex Nees; Laurus malabathrum Wall. ex Nees), and five names are placed into synonymy for the first time. A small number of species are considered to have a Provisional IUCN Conservation Assessment of Least Concern, while most are either Endangered or Critically Endangered, with one species considered to be Data Deficient (Cinnamomum trintaense Kosterm.).

1 ade Kok, Rogier, P.J. uhttps://lauraceae.myspecies.info/node/2039802678nas a2200289 4500008004100000245011500041210006900156300001200225490000700237520182400244653001502068653003202083653000802115653001402123653000902137653000802146653002102154653001602175653001602191653002102207653002202228100002102250700002302271700002902294700001702323856004802340 2019 eng d00aIncongruence of morphological determinations and DNA barcode sequences: a case study in Cinnamomum (Lauraceae)0 aIncongruence of morphological determinations and DNA barcode seq a383-4000 v493 a

In the course of molecular systematic studies of Lauraceae we received a sample of a plant cultivated under the name Cinnamomum porrectum in the Botanical Garden München-Nymphenburg. Preliminary determinations, both morphologically based on the Flora of China (Li & al. 2008) and by chloroplast sequences (psbA-trnH spacer, trnK intron including matK gene, trnL intron, trnL-trnF spacer and trnQ-rps16 spacer) obtained by Sanger sequencing suggested that it was C. camphora, still the plant looked different from other individuals of C. camphora cultivated in the botanical gardens of Berlin, Hamburg, Mainz, Munich and Oldenburg. Attempts to sequence the more informative nuclear internal transcribed spacer repeatedly led to mixed signals. We therefore used Illumina sequencing on a set of pre-amplified molecular markers (ITS, trnK 3′ and 5′ intron, trnL intron, and the intergenic spacers psbA-trnH, trnL-trnF, as well as parts of the trnQ-rps16 spacer), and downloaded available sequences of C. camphora and C. parthenoxylon from GenBank for comparison. Considerable differences were found among these sequences, but the haplotype groups do not coincide with the current species determinations. Particularly the internal transcribed spacer sequences are rather diverse, suggesting possible misidentifications, contaminations, and/or a common gene pool that is larger than anticipated. Concerning the plant in question, our results suggest that it may be a hybrid, with C. camphora as the maternal and another species, possibly C. parthenoxylon, as the paternal parent.

10aCinnamomum10ainterspecific hybridization10aITS10aLauraceae10amatK10aNGS10apsbA-trnH spacer10atrnK intron10atrnL intron10atrnL-trnF spacer10atrnQ-rps16 spacer1 aRohwer, Jens, G.1 aTrofimov, Dimitrij1 aMayland-Quellhorst, Eike1 aAlbach, Dirk uhttps://lauraceae.myspecies.info/node/2039701495nas a2200193 4500008004100000245006500041210006300106260001200169300001200181490000700193520096100200653001101161653001501172653001201187653001401199653001601213100002401229856004801253 2019 eng d00aA new species and new combinations of Neotropical Cinnamomum0 anew species and new combinations of Neotropical Cinnamomum c06/2019 a137-1390 v273 a

A new species of Cinnamomum Schaeff. from the Pacific coast of Ecuador is described and discussed. Cinnamomum latifolium van der Werff is distinctive for its broad triplinerved leaves with canaliculate petioles, densely pubescent young twigs, and 2-locular inner stamens. In addition, six species recently described in Aiouea Aubl. should, in this author’s opinion, be placed within Cinnamomum. The new combinations C.ampullaceum (Lorea-Hern.) van der Werff, C. hirsutum (Lorea-Hern.) van der Werff, C. paratriplinerve (Lorea-Hern.) van der Werff, C. pseudoglaziovii (Lorea-Hern.) van der Werff, C. rubrinervium (Lorea-Hern.) van derWerff, and C. uninervium (Lorea-Hern.) van der Werff are accordingly published. The author citation of the recently transferred C. baitelloanum van der Werff & P.L.R. Moraes to Aiouea is rejected.

10aAiouea10aCinnamomum10aEcuador10aLauraceae10anew species1 avan der Werff, Henk uhttps://lauraceae.myspecies.info/node/2039403558nas a2200217 4500008004100000245011900041210007100160300000900231490000700240520286100247653002003108653001703128653002003145653001303165653001903178100002303197700002303220700002503243700002403268856004803292 2019 eng d00aRiqueza e endemismo de Lauraceae no Paraná: aspectos fitogeográficos e áreas prioritárias para a conservação0 aRiqueza e endemismo de Lauraceae no Paraná aspectos fitogeográfi a1-140 v703 a

Lauraceae está entre as três principais famílias do componente arbóreo das florestas no Paraná, sendo elemento frequente nos estudos fitossociológicos e biogeográficos. Apesar disso, a família continua insuficientemente conhecida no estado. Nossos objetivos foram: reconhecer as espécies de Lauraceae no Paraná, relacionando-as com as unidades fitogeográficas; mapear as regiões com maior riqueza de espécies e concentração de endemismos; identificar áreas de complementaridade e compará-las com as áreas prioritárias para conservação. Para isso, nós utilizamos registros de coleções de 23 herbários nacionais, totalizando 3.332 espécimes analisados, plotando suas coordenadas no mapa fitogeográfico do estado. Constatamos que no Paraná ocorrem 76 espécies, das quais onze são citadas pela primeira vez no estado. A Floresta Ombrófila Densa abrange o maior número de espécies (59 spp.), seguida pela Floresta Ombrófila Mista (31 spp.), Floresta Estacional Semidecidual (23 spp.), Savana (9 spp.) e Estepe (2 spp.). As regiões da Serra do Mar e Vale do Ribeira apresentam a maior riqueza e endemismo. O Parque Estadual das Lauráceas é uma unidade de conservação estratégica, pois abriga dois terços das espécies paranaenses, com sete endêmicas.

Lauraceae are among the three main families of the arboreal component of the forests in Paraná, being a frequent element in phytosociological and biogeographic studies. In spite of this, the family remains insufficiently known in the state. Our aims were: to recognize the species of Lauraceae in Paraná, relating them to the phytogeographic units; map the regions with the greatest species richness and concentration of endemisms; identify complementarity areas and compare them with the priority areas for conservation. We used collection records from 23 national herbaria, totaling 3,332 specimens analyzed, plotting their geographic coordinates onto the phytogeographic map of the state. We found that there are 76 species in Paraná, of which eleven are cited for the first time in the state. The Atlantic Rainforest contains the largest number of species (59 spp.), followed by the Araucaria Rainforest (31 spp.), Semideciduous Forest (23 spp.), Savanna (9 spp.), and Grasslands (2 spp.). The Serra do Mar and Ribeira Valley regions have the highest concentration of species, as well as the highest index of endemism. The Lauráceas State Park is a strategic conservation unit, because it houses two thirds of the species of Paraná, including seven endemic.

10aAtlantic Forest10aBiogeography10acomplementarity10aendemism10apriority areas1 aBrotto, Marcelo, L1 aUhlmann, Alexandre1 aBlum, Christopher, T1 aRoderjan, Carlos, V uhttps://lauraceae.myspecies.info/node/2039200949nas a2200121 4500008004100000245004200041210004000083300001200123490000700135520061300142100002400755856004800779 2019 eng d00aAlseodaphnopsis (Lauraceae) revisited0 aAlseodaphnopsis Lauraceae revisited a186-1890 v643 a

Generic delimitations among the Asian members of the Persea group, including the recently described genus Alseodaphnopsis, are discussed. These genera, with the exception of Alseodaphnopsis, are characterized by reproductive characters. A study of flowers of Alseodaphnopsis species found that Alseodaphnopsis species have unisexual flowers and that Alseodaphnopsis is better defined by its unisexual flowers than by vegetative characters. This is the first report of the presence of unisexual flowers in any member of the Persea group.

1 avan der Werff, Henk uhttps://lauraceae.myspecies.info/node/2039100473nas a2200121 4500008004100000245013600041210007100177260001200248300001200260490000800272100002300280856004800303 2019 eng d00aNotes on Indian Neolitsea (Lauraceae)—two new varietal combinations under N. cassia and N. foliosa and typification of five names0 aNotes on Indian Neolitsea Lauraceae—two new varietal combination c10/2019 a209-2140 v4191 aChakrabarty, Tapas uhttps://lauraceae.myspecies.info/node/2039002752nas a2200241 4500008004100000245007500041210006900116260001200185520205600197653001602253653001302269653001502282653003102297100001302328700001702341700001802358700001802376700001302394700001702407700001402424700002402438856004802462 2019 eng d00aPlastid phylogenomics improve phylogenetic resolution in the Lauraceae0 aPlastid phylogenomics improve phylogenetic resolution in the Lau c08/20193 a

The family Lauraceae is a major component of tropical and subtropical forests worldwide, and includes somecommercially important timber trees and medicinal plants. However, phylogenetic relationships within Lauraceae have long been problematic due to low sequence divergence in commonly used markers, even between morphologically distinct taxa within the family. Here wepresent phylogenetic analyses of 43 newly generated Lauraceae plastomes together with 77 plastomes obtained from GenBank, representing 24 genera of Lauraceae and 17 related families of angiosperms, plus nine barcodes from 19 additional species in 18 genera of Lauraceae, in order to reconstruct highly supported relationships for the Lauraceae. Our phylogeny supports the relationships: sisterhood of the Lauraceae and a clade containing Hernandiaceae and Monimiaceae, with Atherospermataceae and Gomortegaceae being the next sistergroups, followed by Calycanthaceae. Our results highlight a monophyletic Lauraceae, with nine well‐supported clades as follows: Hypodaphnis clade, Beilschmiedia–Cryptocarya clade, Cassytha clade, Neocinnamomum clade, Caryodaphnopsis clade, Chlorocardium–Mezilaurus clade, Machilus–Persea clade, Cinnamomum–Ocotea clade, and Laurus–Neolitsea clade. The topology recovered here is consistent with the patterns of plastome structural evolution and morphological synapomorphies reported previously. More specifically, flower sex, living type, inflorescence type, ovary position, anther locus number, leaf arrangement, leaf venation, lateral vein number, tree height, and inflorescence location all represent morphological synapomorphies of different lineages. Our findings have taxonomic implications and two new tribes, Caryodaphnopsideae and Neocinnamomeae, are described, and the composition of four other tribes is updated. The phylogeny recovered here provides a robust phylogenetic framework through which to address the evolutionary history of the Magnoliids, the third‐largest group of Mesangiospermae.

10achloroplast10aLaurales10amagnoliids10aphylogenetic relationships1 aSong, Yu1 aBin Yu, Wen-1 aTan, Yun-Hong1 aJin, Jian-Jun1 aWang, Bo1 aYang, Jun-Bo1 aLiu, Bing1 aCorlett, Richard, T uhttps://lauraceae.myspecies.info/node/2038900468nas a2200121 4500008004100000245014100041210006900182260001200251300001000263490000800273100001700281856004800298 2019 eng d00aPersea himalayensis (Lauraceae), a superfluous name for Machilus odoratissima and second-step lectotypification of Machilus odoratissima0 aPersea himalayensis Lauraceae a superfluous name for Machilus od c10/2019 a91-940 v4201 aKumar, Anand uhttps://lauraceae.myspecies.info/node/2038802125nas a2200217 4500008004100000245011600041210006900157260001200226300000900238490001000247520144700257653002501704653002801729653001401757653001201771653003001783100001801813700001501831700001301846856004801859 2019 eng d00aPlastome sequences help to improve the systematic position of trinerved Lindera species in the family Lauraceae0 aPlastome sequences help to improve the systematic position of tr c10/2019 a1-170 ve76623 a

Lindera is a genus (c. 100 spp.) of trees belonging to the “core Laureae” group in the family Lauraceae. It is often confused with Litsea, and the systematics of the genus is unclear. Here, total 10 complete plastomes from nine trinerved Lindera species and another species Lindera obtusiloba (sect. Palminerviae Meissn.) were sequenced. Nine highly variable regions, trnH-GUG/psbA, psbM/trnD-GUC, petA/psbL, ndhF, trnL-UAG/ndhD, and ycf1, were identified among the 10 Lindera species. In addition, a total of 1,836 mutation events including six micro-inversions, 156 indels, and 1,674 substitutions, were also summarized. Comparing our sequences with other available plastomes in the “core Laureae,” we put forward that six hypervariable loci, trnH-GUG/psbA, ndhF, ndhF/rpl32, trl32/trnL-UAG, ndhD, and ycf1, could potentially be used as plastid barcode candidates for species identification. Further phylogenetic analyses were conducted using 49 complete Lauraceae plastomes. The results supported a close relationship among trinerved Lindera species and suggested an improved trinerved group comprising species of trinerved Lindera species and Iteadaphne caudate.

10aComparative genomics10aComplete plastid genome10aLauraceae10aLindera10aPhylogenetic relationship1 aTian, Xiangyu1 aYe, Junwei1 aSong, Yu uhttps://lauraceae.myspecies.info/node/2038601755nas a2200277 4500008004100000245009400041210006900135520092700204100001901131700001801150700001501168700001701183700002001200700002001220700001801240700001701258700001801275700001901293700001801312700001801330700001601348700001801364700002701382700002001409856004801429 2019 eng d00aStout camphor tree genome fills gaps in understanding of flowering plant genome evolution0 aStout camphor tree genome fills gaps in understanding of floweri3 a

We present reference-quality genome assembly and annotation for the stout camphor tree (Cinnamomum kanehirae (Laurales, Lauraceae)), the first sequenced member of the Magnoliidae comprising four orders (Laurales, Magnoliales, Canellales and Piperales) and over 9,000 species. Phylogenomic analysis of 13 representative seed plant genomes indicates that magnoliid and eudicot lineages share more recent common ancestry than monocots. Two whole-genome duplication events were inferred within the magnoliid lineage: one before divergence of Laurales and Magnoliales and the other within the Lauraceae. Small-scale segmental duplications and tandem duplications also contributed to innovation in the evolutionary history of Cinnamomum. For example, expansion of the terpenoid synthase gene subfamilies within the Laurales spawned the diversity of Cinnamomum monoterpenes and sesquiterpenes.

1 aChaw, Shu-Miaw1 aLiu, Yu-Ching1 aWu, Yu-Wei1 aWang, Han-Yu1 aLin, Chan-Yi, I1 aWu, Chung-Shien1 aKe, Huei-Mien1 aChang, Lo-Yu1 aHsu, Chih-Yao1 aYang, Hui-Ting1 aSudianto, Edi1 aHsu, Min-Hung1 aWu, Kun-Pin1 aWang, Ling-Ni1 aLeebens-Mack, James, H1 aTsai, Isheng, J uhttps://lauraceae.myspecies.info/node/2038501156nas a2200229 4500008004100000245006200041210006100103260001200164300001200176490000700188520049900195653001700694653002500711653001200736653001100748653001300759100002700772700002600799700002900825700002400854856004800878 2019 eng d00aTwo new species of Lauraceae from Espírito Santo, Brazil0 aTwo new species of Lauraceae from Espírito Santo Brazil c12/2019 a249-2680 v243 a

Two new species from the Brazilian Atlantic rainforest of Espírito Santo State, Licaria spiritusanctensis and Ocotea teresae, are described and illustrated. Their putative relationships within the respective genera are discussed. We also provide illustrations and comments on micromorphological and anatomical features of leaves of these new species, comparing them to those of congenerics and showing that they can be useful for recognition of different taxa.

10aLeaf cuticle10aleaf vascular bundle10aLicaria10aOcotea10ataxonomy1 aMoraes, Pedro, L.R. de1 aFernandes, Tamiris, B1 aAzevedo, Igor, H Freitas1 aBarbosa, Tiago, D M uhttps://lauraceae.myspecies.info/node/2037701637nas a2200241 4500008004100000245013800041210006900179260001200248300001000260490000800270520082900278653003001107653002101137653003201158653002401190653002601214653001401240653002201254100002301276700002701299700002101326856004801347 2019 eng d00aTowards a phylogenetic classification of the Ocotea complex (Lauraceae): classification principles and reinstatement of Mespilodaphne0 aTowards a phylogenetic classification of the Ocotea complex Laur c04/2019 a25-500 v1903 a

The large genus Ocotea (c. 400 species) has been known to be paraphyletic with respect to most other Neotropical genera of Lauraceae for almost 20 years, but a phylogenetic classification has not yet been proposed. Here we present a phylogenetic analysis, based on ITS and psbA-trnH sequences of 123 species from the Ocotea complex, focused on the Neotropical species with bisexual flowers. Our results show that several well-supported clades can be recognized morphologically. We discuss the principles of splitting monophyletic genera from large paraphyletic groups, and we reinstate the genus Mespilodaphne as a first step towards a phylogenetic classification. In addition, three species previously included in Aiouea are transferred to Damburneya.

10aclassification principles10afruit morphology10ainternal transcribed spacer10aparaphyletic groups10aphylogenetic analysis10apsbA-trnH10astamen morphology1 aTrofimov, Dimitrij1 aMoraes, Pedro, L.R. de1 aRohwer, Jens, G. uhttps://lauraceae.myspecies.info/node/20373